Evaluation of Urinary Antigen Detection Methods for Rapid Diagnosis of Legionella pneumonia.
スポンサーリンク
概要
- 論文の詳細を見る
We have evaluated urine specimens of presumptive cases of legionnaires' disease (110 cases, 173 sample), collected in the past eight years (April, 1990-August, 1998) with the Binax EIA kit which detects the soluble antigen of <I>Legionella pneumophila</I> serogroup (SG) 1, and the Biotest EIA kit which detects <I>Legionella</I> species.<BR>Seven cases (19 specimens) were positive for the Binax EIA kit, and nine cases (22 specimens) were positive for the Biotest EIA kit. The sensitivity for culture, PCR, IFA method were 100%, 100%, and 50%, the specificity for these method were 93%, 97.1%, and 90% respectively. Overall agreements for these method were 93.5%, 97.4%, 86.8%, these results suggested that the urinary antigen detection test had high sensitivity and specificity. Our study indicated that concentrated urine samples increase sensitivity.<BR>We also evaluated the capabilities of both EIAs to detect soluble antigens were extracted from bacterial suspension of 18 strains of 5 <I>Legionella</I> species by heating. Both assays detected <I>L. pneumophila</I> serogroups 1 to 14, <I>L. bozemanii</I>. The Binax EIA proved to be useful as the Biotest EIA for diagnosis of legionellosis caused by <I>Legionella</I> species and serogroups other than <I>L. pneumophila</I> serogroup 1. Some cases have been shown to excrete antigen for prolonged period of times despite recovery from infection, so that the patient's history should be sought.<BR>The urine antigen detection EIA methods proved to be rapid and easy to use, detect antigen in the early stage of the desease with high sensitivity and specificity. Its use for the definition of legionellosis should be considered in Japan.
- The Japanese Association for Infectious Diseasesの論文
The Japanese Association for Infectious Diseases | 論文
- Simple Method for Typing Human Immunodeficiency Virus Type 1 (B, E) by PCR and Chronological Change of Distribution ofSubtype (B to E) in Japan.
- Kinetic Analysis of Anti-HIV Titer in Early Stage of HIV Infection. A New Testing Strategy to Differentiate Early HIV Infection Individuals from False Positive Cases.:A New Testing Strategy to Differentiate Early HIV Infection Individuals from False Posit
- HIV-1 (Human Immunodeficiency Virus Type 1) Mutant Strain Associated with Zidovudine Resistance Detected from a Japanese Patient with Primary HIV-1 Infection.
- Epidemiological and Bacteriological Study on Gonococcal Infections.
- A Case of Trichosporon beigelii Peritonitis in CAPD.