Evaluation of a Latex Agglutination Method for Detecting and Characterizing Verotoxin (VT) Produced by Escherichia coli.
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概要
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The detection of VT produced by <I>Escherichia coli</I> is very important for the identification of verotoxin-producing <I>Escherichia coli</I> (VTEC). The latex agglutination reagents (Denka Seiken Co. Ltd, Tokyo) which was developed to detect VT was compared with the vero cell bioassay or polymerase chain reaction method.<BR>A total 147 VT-positive strains (109 serotype O157: H 7/-and 38 non-O157 serotype) and 31 VT-negative strains which were isolated from human were investigated. In addition, a total of 79 VT-positive strains (14 serotype O157: H7i and 65 non-O157 serotype) and 79 VT-nagative strains which were isolated from animals were also examined<BR>The latex agglutination assay for the human isolates showed the 100% sensitivity, specificity and agreement. The assay for the animal isolates showed 94.9% sensitivity, 100% specificity and 97.5% agreement. Although 4 of 8 strains isolated from swine which produce VT2 variant toxin (VT2e) failed in detecting verotoxin by latex agglutination assay, VT2e was not related to human infections.<BR>We conclude that this latex agglutination reagent is highly sensitive and specific for detecting and characterizing VT of <I>E. coli</I>. The method is reliable, easy to perform at any laboratories.
- The Japanese Association for Infectious Diseasesの論文
The Japanese Association for Infectious Diseases | 論文
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