Detection of the trh Gene in Vibrio parahaemolyticus Isolated from Overseas Travellers' Diarrhea and Their Biochemical Characteristics.
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概要
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A total of 478 <I>Vibrio parahaemolyticus</I> strains isolated from overseas travellers' diarrhea during the last 7 years of 1989 to 1995 were examined for the production of Kanagawa hemolysin by reversed passive latex agglutination (RPLA) test. Three hundred-seventy (77.4%) out of 478 strains were positive for Kanagawa hemolysin, whereas 108 strains were weakly positive or negative.<BR>For those Kanagawa hemolysin-weakly positive or nagative strains, the <I>tdh</I> and <I>trh</I> genes associated with the production of TDH (thermostable direct hemolysin) and TRH (TDH-related hemolysin), respectively, were studied by the polymerase chain reaction method. The <I>trh</I> gene was detected in 98 (90.7%) out of 108 strains. In 35 strains belonging to 13 serotypes such as 03: K6, O1: K33, O3: K59, the <I>trh</I> gene alone was detected. On the other hand, both <I>trh</I> and <I>tdh</I> genes were detected in 63 strains of 17 serotypes including O1: K69, O3: K72, O6: K46. Among the strains of 4 serotypes including O1: K56, O1: KUT, O3: KUT and O5: KUT, two types of the <I>trh</I> positive alone and the <I>trh</I> and <I>tdh</I> positive were observed.<BR>Of interest, all of the 98 <I>trh</I>-positive strains were positive for the urease hydrolysis, whereas all Kanagawa hemolysin-positive strains were not. Furthermore, the strains of serotype O6: K18 (4 strains) were positive for the fermentation of dulcitol, and the strains of serotype O1: K1 (5 strains) were indole negative. These characteristics of the strains were completely different from those of typical <I>V. parahaemolyticus</I> strains.
- The Japanese Association for Infectious Diseasesの論文
The Japanese Association for Infectious Diseases | 論文
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