Isolation and Incidence of Vibrio cholerae from River Water.
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概要
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The prevalence of Vibrio cholerae contamination in river water derived from 20 sites of 18 rivers in Kanagawa, Japan, was investigated during a period from July to September, 1987, and from one of the 20 sites in August, 1988 and in February, 1989. <I>V. cholerae</I> non-O1 was found in all samples at concentrations of 0.9->1, 400 MPN/100 ml. Higher amounts of the organism were observed in the samples from estuaries. <I>V. cholerae</I> O1 was detected in samples collected in August, 1988 and in February, 1989 at concentrations of 150 MPN/100 ml and 1.5 MPN/100ml, respectively. From 1989 to 1995, water samples were collected monthly from 10 sites of 10 rivers to detect <I>V. cholerae</I>. <I>V. cholerae</I> O1 and non-O1 were detected in 3.6%(30 of 840) and in 61.1%(513 of 840) in the water samples examined, respectively. Overall, <I>V. cholerae</I> was found in 62.9%(528 of 840). Both types, O1 and non-O1, of organisms were detected in 15 samples. These results indicated that river water was contaminated frequently with <I>V. cholerae</I> non-O1 and sporadically with <I>V. cholerae</I> O1 throughout the year. Only one strain of <I>V. cholerae</I> O1 out of 543 <I>V. cholerae</I> strains was found to be a producer of cholera toxin. During these studies, the selectivity of 3 media for <I>V. cholerae</I> O1 was evaluated, and PMT agar was found to be the best.
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The Japanese Association for Infectious Diseases | 論文
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