Pillin Structural Gene and Bacterial Adhesion to Cultured Cell of Pseudomonas aeruginosa Isorated from Clinical Materials.
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概要
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Thirty-three stocks of <I>Pseudomonas aeruginosa</I> which is an important etiologic agent of opportunistic infecitons were clinically isolated. The pillin structural gene <I>pil</I>A of the stocks were amplified by polymerase chain reaction (PCR) and classified into 3 groups; 2000 bp (16 stocks; 48.5%), 1300 bp (11 stocks; 33.3%), 550 bp (6 stocks; 18.2%). The adhesiveness of the stocks to cultured human lung cancer origin calu-1 was also determined, their adhesion rate per cell were 39.2%, 24.8%, 22.1% in average respectively. Thus clinically most common 2000 pb group is remarkably easier to adhere to calu-1. Serotypes of the strains were examined to reveal the difference of the distribution that F, G, I types were dominant in 2000 pb group, but E types were major in 1300 bp and 550 bp groups. These data sugest that the gene arrangement of <I>pil</I>A influences adhesiveness to cultured cell and antigenicity of bacteria.
- The Japanese Association for Infectious Diseasesの論文
The Japanese Association for Infectious Diseases | 論文
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