Detection of Pseudomonas aeruginosa antigens in sputum, broncho-alveolar lavage and concentrated urine by enzyme-linked immunosorbent assay.
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A sandwich enzyme-linked immunosorbent assay (ELISA) for detection of a 11 serotypes of <I>P. aeruginosa</I> has been developed by using polyclonal rabbit anti P. aeruginosa (NC-5 strain) antiserum. The following results were obtained:<BR>1) Cultured broth of <I>P. aeruginosa</I> was detected most sensitively by this assay followed by sonicated soluble antigen, washed bacetrial fluid and heated soluble antigen of <I>P. aeruginosa</I>. The lower limit of detection was approximately 2.3 × 10<SUP>4</SUP> cfu/ml of <I>P. aeruginosa</I> (NC-5 strain) (containing 22.2 ng/ml of protein) in cultured broth.<BR>2) By this assay, all serotypes of <I>P. aeruginosa</I> and some strains of Family Pseudomonadaceae (RNA group I) were detected, but no cross reaction was noticed to other species of bacteria (24 species, 268 strains).<BR>3) The serum antigen in the experimental sepsis of <I>P. aeruginosa</I> or murine experimental pneumonia was detected when at least approximately 10<SUP>3</SUP> cfu/ml of <I>P. aeruginosa</I> was present in serum or BALF. But the antigen was detected from concentrated urine even under the concentration of 10<SUP>2</SUP> cfu/ml in sepsis.<BR>4) <I>P. aeruginosa</I> antigens in sputum of patients with chronic respiratory tract infection was also detected. There was a significant difference between absorbance value of sputum in non-infected patients (group 1) and one in infected patients by <I>P. aeruginosa</I> (group 3) (p<0.01), and between one in infected patients by bacteria other than <I>P. aeruginosa</I> (Group 2) and one in Group 3 (p<0.01). And there was a strong correlation between absorbance value of sputum in Group 3 and number of <I>P. aeruginosa</I>.<BR>In 22 of 33 sputa (66.7%) with <I>P. aeruginosa</I> infection as verified by conventional culture methods, the antigen was detected in sputum. In a patient of <I>P. aeruginosa</I> pneumonia with acute leukemia, the antigen was detectable from spurum and one of the series of concentrated urine. It is concluded that the detection of <I>P. aeruginosa</I> antigen in sputum and urine by ELISA is a helpful aid to diagnose <I>P. aeruginosa</I> infection.
- The Japanese Association for Infectious Diseasesの論文
The Japanese Association for Infectious Diseases | 論文
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