Diagnosis of enterotoxigenic Escherichia coli by hydrophobicity test using salting out.
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概要
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The value of hydrophobicity test based on salting out was examined by using Escherichia coli isolated from traveller's diarrhea in order to diagnose enterotoxigenic <I>E. coli</I>. In all, 821 <I>E.coli</I> isolates from 180 diarrheal patients were tested and the following results were obtained.<BR>1) Out of 157 strains of positive hydrophobicity test, 137 and 20 strains were enterotoxigenic and non-enterotoxigenic <I>E. coli</I>, respectively.<BR>2) Out of 664 strains of negative hydrophobicity test, 29 and 635 strains were enterotoxigenic and non-enterotoxigenic <I>E.coli</I>, respectively.<BR>3) Among strains of positive hydrophobicity test, 54, 17 and 66 produced only heat-stable enterotoxin (ST), only heat-labile enterotoxin (LT) and both ST and LT, respectively.<BR>4) Screening efficiency for identifying enterotoxigenic <I>E.coli</I> by salting out test was 83% in sensitivity and 97% in specificity.<BR>These data suggest that the hydrophobicity test using salting out is a rapid, inexpensive, and simple screeng test for enterotoxigenic <I>E. coli</I>.
- The Japanese Association for Infectious Diseasesの論文
The Japanese Association for Infectious Diseases | 論文
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