:The First Report Virus Isolation Employing a New Microplate Method
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概要
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To simplify the procedure of virus isolation, a new microplate method has been attempted. Four kinds of cell, i.e. human embryonic fibroblast (HEF), Hep-2, Vero, MDCK were prepared together in a tissue culture microplate. 657 viruses recovered from throat swabs obtained from 2, 310 infants and children with acute respiratory infections (ARI) tested between December, 1984 and December, 1985. The isolates were influenza viruses, RS virus, parainfluenza viruses, mumps virus, adenoviruses, enteroviruses, herpes simplex virus and cytomegalovirus. Influenza and parainfluenza viruses were isolated by adding trypsin to a medium. The new microplate method is more sensitive to virus isolation, simpler to handle, cheaper economically and more convenient than ordinary tube method for the isolation of viruses from syndrome due to multiple agents such as ARI.
- The Japanese Association for Infectious Diseasesの論文
The Japanese Association for Infectious Diseases | 論文
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