Whole blood chemiluminescence response and virulence of various organisms by systemic infection.
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概要
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The phagocytic cells such as leukocytes and macrophages emit light or chemiluminescence during phagocytosis. The chemiluminescence response of mouse whole blood was studied for <I>Escherichia coli</I>, <I>Klebsiella pneumoniae</I>, and <I>Pseudomonas aeruginosa</I>. We compared the chemiluminescence response and the superoxide dismutase (SOD) of organism, the phagocytosis of organism by using mouse neutrophils, and the virulence of organisms by mouse systemic infection. The results may be summarized as follow: The slope of the initial chemiluminescence and maximum chemiluminescence was independent of the test organism. The organisms which showed the high chemiluminescence response were low SOD activity, sensitive for killing by neutrophils, and high minimum lethal dosis. The whole blood chemiluminescence assay is a simple, sensitive method of evaluating the function of relatively small numbers of phagocytic cells. The chemiluminescence response of whole blood may be a reliable assay for virulence of organism.
- The Japanese Association for Infectious Diseasesの論文
The Japanese Association for Infectious Diseases | 論文
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