Production of leukocidin of Pseudomonas aeruginosa strains isolated from patients and environmental specimens.
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概要
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A ratio leukocidin-producing strains to clinical isolates was investigated together with production of protease, elastase and exotoxin.It was also examined whether these strains contain the common proteineous antigen (OEP) which resides in the cell wall.By the agar gel precipitin test using crystalline or highly purified antigens, leukocidin, protease, elastase, exotoxin and OEP, and their specific antisera, it was proved that leukocidin reacted only against the homologous serum.Similarly, the serological specificity of the other four antigens was also confirmed.<BR>In order to detect production of leukocidin a strain to be tested was inoculated on one side of the Petri dish which was incubated at 37°C for 2 days and exposed to chloroform vapour.Then 5 wells were made at a distance of 5 mm from the growing culture, and these were filled with the 5 kinds of antisera. Precipitin reaction was judged after the dish was left at 25°C overnight.Consequently, 87 clinical isolates (96.7%) out of 90 were found to produce leukocidine.Protease, elastase and exotoxin were also prodused in high percentages.OEP was found to exist in all strains.
- The Japanese Association for Infectious Diseasesの論文
The Japanese Association for Infectious Diseases | 論文
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