Identification of Neisseria Gonorrhoeae by Co-agglutination Test
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概要
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A slide co-agglutination test was perfomrd, using a serological identification reagent "Phadebact <SUP>®</SUP> Gonococcus Test"(Pharmacia Diagnostic AB: Sweden) with 120 strains of Neisseria gonorrhoeae, 42 strains of N. meningitidis, 19 strains of other Neisseria species, 6 strains of <I>Branhamella catarrhalis</I> and 2 strains of <I>Haemophilus influenzae</I>.<BR>The Menck's direct colonies method, by which a few trypsin treated colonies of organisms grown on serum-free medium were mixed directly with the reagent, produced 10% of non-interpretable results among 80 strains of <I>N. gonorrhoeae</I>. All of the non-interpretable were predominated with T2 colony. In addition 11% of <I>N. gonorrhoeae</I> produced negative results by this method, and all the neagative strains were constituted of T4 colony only.<BR>On the other hand, a new procedure of boiling colonies method, described by the manufacturer, by which the organisms suspended in distilled water were heated at 100°C for 5 min prior to testing, produced positive results in all strains of <I>N. gonorrhoeae</I> examined. No positive results were obtained with 69 sti ains of non-gonococcal isolate by either method. However the number of non-interpretable was reduced and that of negative was increased by boiling colonies method.<BR>Using 120 strains of <I>N. gonorrhoeae</I> a comparison was made between co-agglutination (COA) test and conventional biological identification test by the Cystine Trypticase Agar (CTA) sugar degradation procedure. All strains of <I>N. gonorrhoeae</I> produced positive results by the COA test, while 2 strains 1.7% did not degrade glucose by the CTA procedure.<BR>The fresh clinical isolates as well as stock strains of <I>N. gonorrhoeae</I> produced positive results by the COA test, excepting for some few strains constituting of T4 colony only which produced somewhat weak postitive reaction.<BR>Using the colonies of oxidase positive and Gram-negative cocci primarily isolated on selective medium, or colonies transferred on non-selective media, a rapid identification by COA test alon was possible, except for a few non-gonococcal colonies with non-interpretable results which needed biological identification.<BR>Though there are some problem with respect to price and supply route for the reagent, the COA test is considered to be a very convenient, practical and accurate serological identification method for<I> N. gonorrhoeae</I> even for small bacteriological laboratories.
- The Japanese Association for Infectious Diseasesの論文
The Japanese Association for Infectious Diseases | 論文
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