Experimental Salmonellosis in Cynomolgus Monkey:I. Studies on Immune Globulins of Cynomolgus Monkey and its Isolation Methods
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To contribute to the clarification of the developmental process of human intestinal infections, experimental studies were done producing salmonellosis on monkeys (cynomolgus). And we are reporting a series of experiments and in this paper isolations of immune globulins from the serum and intestinal tissues of the monkeys were performed as the preliminary experiments.<BR>Results were summarized as follows:<BR>I) Monkey IgM, IgG, and IgA were cross-reacted with human IgM, IgG, and IgA, respectively, and the common antigens were recognized, respectively.<BR>II) Separation of IgM: Precipitates of the whole serum by 33% Am Sul were fractioned by gelfiltration on the column of Sephadex G-200, and effluents were separated into two peaks (18s and 7s), and IgM was obtained by zone electrophoresis in 18s fraction.<BR>III) Separation of IgG: Above obtained 7s peak was separated by DEAE chromatography using 0.01 M PH 7.4 PBS.<BR>IV) Separation of IgA: From the extracts of monkey intestine tissue, y fraction was obtained by zone electrophoresis. This fraction was chromatographed on DEAE-cellulose, and the effluent obtained through PH 6.4, 0.10 M was further refined through Sephadex G-200 gel.<BR>V) H.L. chain separation from IgG: Refined IgG was reduced by 2-ME and alkylated by iodoacetamide, and then geI-filtrated through Sephadex G-200 in 1 N H. Ac solution at the room temperature. Heavy and light chain were thus separated. Proportion of H and L chain was 74: 26.
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