New Method for Assaying Free and Total Cholesterol in Cultured Cells by High-pressure Liquid Chromatography
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We developed a simple, sensitive and accurate method for assaying cellular free and total cholesterol by monitoring 4-cholesten-3-one, a conversion product of the cholesterol oxidasecatalyzed oxidation of the free cholesterol that has a strong chromophoric α, β-unsaturated ketone at 20 nm, using a high-pressure liquid chromatographic system. This method measured picomole quantities of free and total cholesterol and precisely determined their concentrations in cells (10<SUP>4</SUP> range) in culture using 7β-hydroxycholesterol as an internal standard. <I>J Atheroscler Thromb, 1996 ; 2 : 117-121.</I>
- 一般社団法人 日本動脈硬化学会の論文
一般社団法人 日本動脈硬化学会 | 論文
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