Amino acid composition of <I>Aphelenchoides besseyi</I>
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概要
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Amino acid composition of <I>A. besseyi</I> propagated aseptically on alfalfa callus tissues was investigated. The nematode material comprising larvae and adults was homogenized and then separated into two parts by centrifugation; one is supernatant containing free amino acid, and the other is sediment containing tissue protein. Then, the sediment was hydrolysed to amino acid. The amounts of amino acids in the supernatant and the hydrolysate were measured with an amino acid autoanalyser. Twenty-five kinds of amino acids, 4 amins, urea, ammonia and 5 unknown ninhydrin positive compounds were detected. Of the amino acids detected, Alanine, Glutamine, Glutamic acid and Glycine from the supernatant and Glycine, Glutamic acid, Aspartic acid, Alanine, Leucine, Proline, Lysine, Valine and Serine from the hydrolysate were abundant. Analytical method of the amino acid and relation between amino acid composition and the metabolism were discussed.
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日本線虫学会 | 論文
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