Freeze-thaw Survival of the Entomogenous Nematodes, Steinernema feltiae and S. glaseri in Brief Comparison with Survival of Caenorhabditis elegans and Aphelenchus avenae

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Survival in liquid nitrogen was investigated for the infective juveniles of entomogenous nematodes Steinernema feltiae and S. glaseri, dauer juveniles of free-living nematode Caenorhabditis elegans, and adults of mycophagous nematode Aphelenchus avenae. When the frozen nematodes were quickly thawed at 35°C, the survival rate was over 90% for S. feltiae, 50% for A. avenae, 30% for C. elegans and less than 1% for S. glaseri. Glycerol was a more effective freeze protectant than dimethyl sulfoxide, however, the latter effectively protected nematodes frozen at -80°C. Irrespective of freeze protectants used, survival was always poor for the nematodes thawed slowly at room-temperature. Jpn. J. Nematol. 18: 30-35 (1989).
日本線虫学会の論文