Microalgae culture system for CO2 elimination and O2 regeneration in the closed system

概要

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We have investigated microalgae culture systems for CO2 elimination and O2 regeneration in the closed system containing low CO2 concentration (800ppm to 1%). A stainless steel reaction vessel (20l) with fluorescent lamps (20W×11) was constructed. We have already studied the effects of temperature, pH, O2 concentration in supply gas using the reactor aerated with low CO2 concentration on the cell growth of Chlorella sorokiniana ATCC22521 and the CO2 elimination rate in the reaction vessel. CO2 elimination and O2 regeneration were investigated with Chlorella sorokiniana ATCC-22521 under two light energy conditions (using 20W×11 fluorescent lamps and 20W×6 fluorescent lamps). As the result, both cell growth of Chlorella and CO2 elimination rate under the high light energy condition were higher than that under the low light energy conditions. But in comparison to the photosynthetic activity based on the chlorophyll content in the Chlorella cell was almost the same under two lighting condition. We further investigated culture conditions for operating the system continuously with a half-batch culture. We confirmed that the microalgae culture system maintained the ability of continuous CO2elimination and O2 generation when cell number and the concentration of nitrogen source in the medium were kept under the adequate conditions. However bacterial contamination in the vessel inhibited of the CO2 elimination.
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