Assessment of Gene Associated with Streptococcus mutans Biofilm Formation
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概要
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<I>Streptococcus mutans</I>, the primary etiological agent of human dental caries and an obligate biofilmforming bacterium, has developed a variety of mechanisms to colonize the tooth surface. Oral transmission of <I>S. mutans</I> through contact between mother and child is thought to be one of the risks of developing dental caries. In this study, I surveyed oral transmission frequency of <I>S. mutans</I> from mother to a 3-year-old child. In 19 (10.9%) of 174 mother-child pairs, <I>S. mutans</I> was isolated from both mother and chihld. The identities of genomic DNA from <I>S. mutans</I> in 5 (45.5%) of the 11 mother-child pairs were presented. Among those, the biofilms formed by <I>S. mutans</I> 3 c and 4 c, respectively, which had high and low ability of biofilm formation, were examined by confocal laser scanning microscopy (CLSM) . Microscopic analysis revealed that the volume of biofilm formation in 3 c was higher than that in 4 c biofilm in the bottom. Furthermore, DNA microarrays were used to study the gene expression profiles of 3 c and 4 c biofilms. In this paper we describe that about 3.8% of genes showed differential expression ; about 2.2% of genes were activated and about 1.6% were repressed in 3 c biofilm compared with 4 c biofilm. The present study suggests that biofilm gene expression is strongly associated with differential biofilm formation. Our identification of biofilm-involved genes points to mechanisms of the virulence of <I>S. mutans</I> and provides a first foothold for studying the natural history of <I>S. mutans</I> infections in mother and child.
- 口腔病学会の論文
口腔病学会 | 論文
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