Studg on radioimmunoassay of FDP
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概要
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Fibrinogen degradation products, fragment D (FgD) and fragment E (FgE) were measured in human serum by an radioimmunoassay (RIA) technique.Fibrinogen was digested for five hours by plasmin to obtain FgD and FgE as the major products.Techniques for separating these fragments from each other and from larger fragments (FgX, FgY) present in the crude digest, comprise gelfiltration with Sephadex G-200 and isoelectric fractionation which gave satisfactory purity and efficiency for preparative purpose.Fragments were iodinated by chloramin T method, and separation of antibody bound and free antigen was achieved using second antibody.In eighteen healthy subjects, the serum level of FgE ranged from 104 to 206ng/ml and raised levels were found in the patients with disseminated intravascular coagulation in the course of acute promyelocytic leukemias and gastric cancers.Advantages of RIA system over the tanned red cell haemagglutination inhibition immunoassay was its higher sensitivity (20ng/ml)There is, however, a possibility that RIA of of FgDP is detecting residual fibrinogen in the serum, since antiFgDP antiserum showed weak cross-reaction to fibrinogen by double immunodiffusion test.In order to eliminate interference by residual fibrinogen in the serum, antiserum against FgDP was further purified by affinity chromatography to remove antifibrinogen activity.A radioimmunoassay, using this FgDP specific antiserum is now under investigation.
- 一般社団法人 日本血栓止血学会の論文
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