Platelet adhesiveness and aggregability
スポンサーリンク
概要
- 論文の詳細を見る
Platelet adhesiveness and aggregability were studied comparatively using human citrated blood.1) Adhesiveness in glass beads (Hellem I) was compared to the intensity of platelet aggregation as measured concurrently by the optical density (O. D.) method using ADP, adrenaline and collagen in 25 patients in convalescence of various diseases. No significant correlation between two test results was found (Fig. 1).2) Citrated blood (0.38%) was filtrated through glass filter (glass beads column or glass-wool braid) repeatedly (up to 4 times) with the filter renewed each time. Platelet count (by Coulter Counter) and platelet aggregability were assessed before and after filtrations. Platelet aggregability was measured by O. D. method. the screen filtration pressure (SFP) method and a method for assessment of platelet sensitivity to ADP-aggregation.i) Platelet count decreased significantly (P<0.01-0.05) after each filtration.ii) The intensity of platelet aggregation measured by O. D. method 5 minutes after addition of ADP (10μM, final concentration) or SFP method using 3μM ADP did not change sigificantly after filtrations when the platelet numbere of the pre-filtration and post-filtration samples were equalized using platelet-poor plasma (PPP) (Fig. 2 and Fig. 3).iii) The SFP value decreased significantly (P<0.01) after filtration when the platelet numbers of the pre-filtration and post-filtration samples were not equalized (Fig. 4).iv) Platelet sensitivity to ADP-aggregation of the platelets in the pre-filtration and post-filtration samples was assessed by a new device (Sano et. al. 1974). Platelet-rich plasma (PRP) was separated without centrifugation. Then the PRP and serially two-fold diluted ADP solution (2-5-2-15mg/ml) were mixed. The minimum effective ADP concentration to give platelet aggregation was detected microscopically. The sensitivity increased after each filtration in both of the observations with and without platelet number equalization (Fig. 5).v) It was suggested that the degree of platelet injury may be reflected more on the platelet sensitivity to ADP-aggregation than on the intensity of platelet aggregation.
- 一般社団法人 日本血栓止血学会の論文
一般社団法人 日本血栓止血学会 | 論文
- 日本人のＡＤＡＭＴＳ１３
- Infection of specific strains of Streptococcus mutans exacerbated intracerebral hemorrhage
- Transcriptional regulation of megakaryopoiesis and thrombopoiesis
- 新規血小板活性化受容体ＣＬＥＣ－２ その発見から今後の展望まで:その発見から今後の展望まで
- １．臨床血栓止血学オーバービュー