Effects of angiotensin - I converting enzyme related substances upon the prostacyclin generation in cultured human vascular endothelial cells with special reference to the kinetics of Ca++.
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The effects of angiotensin I (AI) converting enzyme (ACE) related substances upon prostacyclin (PGI2) generation with special reference to the kinetics of Ca++ were investigated using cultured human vascular endothelial cells.Endothelial cells were isolated from human umbilical cord vein and cultured by the modified method of Jaffe et al. PGI2 generation or Ca++ kinetics were measured using 6-keto PGF1α RIA assay kit or 45Ca, respectively.AI or bradykinin (BK) (10-6M) increased PGI2 generation, however captopril decreased it. On the other hand angiotensin II (AII) did not show any effect. The enhanced PGI2 generation by AI or BK was remarkably decreased by the pretreatment with an intracellular Ca++ immobilizer, TMB-8 (10-4M). AT and BK enhanced 45Ca uptake, while AII did not show any effect on it, and captopril decreased it.These results suggested that PGI2 generation by ACE related substances were closely linked to the Ca++ uptake to the cytosol from extracellular space or to the release of Ca++ from the storage site.
- 一般社団法人 日本血栓止血学会の論文
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