Morphological analysis of platelets adhering to polymer surfaces with functional groups: A scanning and transmission electron-microscopic study.:A scanning and transmission electron-microscopic study
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Progress in implantable artificial organs such as the artificial heart, artificial vessele and the artificial kidney depends greatly on the development of excellent antithrombogenic materials because prevention of thrombosis is indispensable for the successful utilization of these artificial organs. In order to design excellent antithrombogenic materials, it is important to clarify the effects of the functional groups of the material surfaces on the surface-induced activation of platelets.For this purpose, poly (styrene) (PSt) (nonionic, hydrophobic polymer), and poly (p-dimethylaminoethylstyrene) (PMAS) (weakly cationic polymer) were prepared, and the adhesion of rat platelets to these polymer surfaces was examined. Albumin-coated as well as non-coated polymer surfaces were used in this experiment.Platelets were collected from 5 rats by cardio-puncture, and were suspended in Hanks' balanced salt solution (Ca2+, Mg2+ free). The interaction of the platelets thus prepared with the polymer surfaces was studied by the microsphere column methed at 4°C and at room temperature. The morphological changes of the adhering platelets on the polymer surfaces were observed by scanning (SEM) and transmission electron-microscopes (TEM).Platelets adhered to the albumin-coated as well as non-coated PMAS surfaces at room temperature; they were rough-surfaced, round in shape with a few long pseudopods. TEM observation revealed that the platelets on these PMAS surfaces had retained more organella such as α-granules than those on the non-coated PSt surface, which had a spreading form. Rounded platelets were observed on the PSt surface with albumin-coating. Similar results were obtained for experiments done at 4°C. Despite their distinct morphology, there was no significant difference in the percent retention of platelets between PSt and PMAS.Thus, it is suggested that cytological changes of the platelets do not always coincide with adhesivity of the platelets to the material surface. Consequently, this study revealed that the albumin-coated as well as non-coated PMAS surfaces with a weakly cationic amino group have a high adhesivity to platelets but have lower tendency to activate platelets than the nonionic non-coated PSt surface.
- 一般社団法人 日本血栓止血学会の論文
一般社団法人 日本血栓止血学会 | 論文
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