Analysis of platelet membrane GPIIb-IIIa using flow cytometry.

概要

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Platelet membrane glycoprotein (GP) IIb and IIIa form a Ca2+-dependent, heterodimer complex (GPIIb-IIa complex). Monoclonal antibodies that are specific for the platelet membrane glycoprotein IIb-IIIa complex bind to resting platelets, but will not bind to platelets that have been exposed to EDTA at 37°C. We developed new monoclonal antibodies (NNKY1-32, 2-6, 2-18) that react with a determinant specific to the GPIIb-IIIa complex, and we studied the distribution of GPIIb/IIIa on platelet surface in the living body and some factors that have influence on the structual of GPIIb/IIIa using flow cytometry. The results obtained from this study indicated following facts. 1. The surface of large platelets is richer in GPIIb/IIIa than that of small platelets. 2. The incubation of intact platelets with EDTA at 37°C causes the progressive dissociation of GPIIb-IIIa complexes, and this influence is marked in small platelets. 3. The addition of Ca2+ or Mg2+ in the reversible dissociation of GPIIb-IIIa complexes causes the reassociation of GPIIb and GPIIIa, and this reassociation is even more marked after ADP stimulation. 4. The prolonged incubation of intact platelets with EDTA at 37°C causes GPIIb and GPIIIa to form peculiar complexes different from GPIIb/IIIa complexes in intact platelets.
一般社団法人日本血栓止血学会の論文