Conversion of Glu-plasminogen to Lys-plasminogen : Analysis by immunoblotting.:analysis by immunoblotting
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When Clu-plasminogen (pig) was incubated with various units of plasmin for 60min and 120min at 37°C in the presence or absence of fibrin, Glu-plg was hardly converted to modified (Lys) pig by up to 0.1cu/ml plasmin in the absence of fibrin. On the other hand, Glu-pig was significantly converted to Lys-plg by plasmin in the presence of fibrin. Increase in the amounts of plasmin used resulted in more conversion of Glu-plg to Lys-plg in the presence of fibrin. When human plasma or clotted plasma was activated by urokinase (UK), Glu-plg was activated better to plasmin by UK in the presence of clot. The presence of fibrin resulted in faster conversion of Glu-plg to Lys-plg by plasmin in both purified and plasma systems.
- 一般社団法人 日本血栓止血学会の論文
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