Endotoxin-induced DIC and tissue thromboplastin activity of bone marrow cells.

概要

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1. Endotoxin injection caused significant increase in procoagulant activity (PCA) of bone marrow cells. Endotoxin also caused an increase in PCA of supernatant (sup) obtained from bone marrow suspension.2. Both activities were destroyed by heating at over 56°C. The activity of mouse brain thromboplastin used as reference preparation was destroyed at 60°C 'but stable at 80°C.3. PCA of bone marrow cells decreased after incubation with Concanavalin A (Con A). The activity recovered by addition of α-methyl-D-glucoside.4. Ploymyxin (PxB) inhibited the PCA of either cells (20%-inhibition) or sup (50%-inhibition).5. Addition of phospholipid (PL) to the cells or sup caused enhancement of PCA.6. Degrees of heat inactivation, Con A- or PxB-inhibition, and PL-enhancement of PCA were higher in sup than those in the bone marrow cells.Procoagulants of the cells and sup were shown to be tissue thromboplastin (Tp) which consists of glycoprotein-phospholipid complex functioning as active sites. Membrane perturbation due to endotoxin would initiate the increase in exposure and/or contents of the active site (PL) on the membrane surface and would participate in the manifestation of Tp activity. As a result of membrane alteration, release of Tp from the cell surface into circulation may also be caused in endotoxemia.
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