タイトル無し

概要

論文の詳細を見る
In the preliminary repory, we have demonstrated that the supernatant obtaind by centrifugation at 10, 000xg from human placental extracts shows a potent inhibitory effect on platelet aggregation, and that placental protein (PP5; Bohn, 1977) has also an inhibitory effect of platelet aggregation as well. In this report, the two contrary effects on platelet aggregation were found in the process of the purification of platelet antiaggregatory substances from human placental extracts.Results; 1) The supernatant obtained at 10, 000xg for 15min, and the precipitate obtained at 104, 000xg for 60min. from human placental extracts showed the strong inhibitory effects on platelet aggregation which was prominent in the precipitate of microsomal fraction. 2) A definite fraction of the supernatant obtaind at 104, 000xg for 60min, after gel-filtrayion showed an acceleration of platelet aggregation. 3) Although PP5 was demonstrated in the supernatants at 10, 000xg for 15min. and at 104, 000xg for 60min. by the use of an immunoperoxidase technique, PP5 was not found in the precipitate at 104, 000xg for 60min.In conclusion, it is evident that there are least two contrary substances showing inhibition and stimulation on platelet aggregation in human placental extracts.
一般社団法人日本血栓止血学会の論文