:Detection of glucose-6-phosphatase activity
スポンサーリンク
概要
- 論文の詳細を見る
It is sometimes difficult to distinguish endoplasmic reticulum (ER) from platelet demarcation membranes (PDM) in megakaryocyte. We utilized the glucoes-6-phosphatase (G-6-Pase) reaction to mark ER at the ultrastrural level. Aspirated bone marrow fragments of an idiopathic thrombocytopenic purpura case were exmined. The modified Wachatein-Method was used for the detection of G-6-Pase activity. The bone marrow fragments were fixed in a 2% glutaraldehyde solution with 0.1M cacodylate buffer (pH7.4) for 10min. at 0-4°C. After rinsing in 0.1M cacodylate buffer, the fragments were incubated in a medium containing 0.375% G-6-Pate, 20ml; 0.2M tris-maleate buffer (pH6.7), 20ml; 2% lead nitrate, 3ml saccharose, 3.0g and H2O 7ml (G-6-P medium), for 1 hour at room temperature. After rinsing again, the fragments were post-fixed. Ultrathin sections were unstained with lead citrate, or double stained with uranyl acetate and lead citrate prior to examination. Lead phosphate deposits, specifically indicating sites of G-6-Pase activity, were present in the perinuclear space and in the saccules of ER. However, deposites did not appear in the PDM or the single-membrane organelles. We could thus easily distinguish ER from PDM.This is the first time that G-6-Pase activity has been utilized to mark ER in human megakaryocyte. We have demonstrated that G-6-Pase activity can be used to investigate the ultrastructure and function of megakaryocyte.
- 一般社団法人 日本血栓止血学会の論文
一般社団法人 日本血栓止血学会 | 論文
- 日本人のＡＤＡＭＴＳ１３
- Infection of specific strains of Streptococcus mutans exacerbated intracerebral hemorrhage
- Transcriptional regulation of megakaryopoiesis and thrombopoiesis
- 新規血小板活性化受容体ＣＬＥＣ－２ その発見から今後の展望まで:その発見から今後の展望まで
- １．臨床血栓止血学オーバービュー