タイトル無し
スポンサーリンク
概要
- 論文の詳細を見る
We have examined morphological and biochemical changes in membrane surface components of platelets during thrombin induced aggregation. To washed human platelets suspension (0.5×109/ml), bovine thrombin was added at concentration of 0.5 NIH units/ml and the mixture was stirred at 37°C for various period of time. Thrombin treated and control (non-treated) platelets were then subjected to electron microscopic observation, lactoperoxidase iodination and SDS polyacrylamide gel disc electrophoresis for glycoprotein analysis. Electron microscopic observation of platelet-aggregates formed 3min. after addition of thrombin demonstrated that there were irregularly spaced bridges of amorphous and filamentous substances between closely approximated plasma membranes. These interplatelet connection reacted with ruthenium red and was thought to be derived from membrane surface. Ten min. after adding thrombin, there found a distinct contact between outer leaflets of membranes of platelets and partial fusion of the membrane was observed.Iodine labelling pattern in membrane polypeptides of thrombin treated platelets showed a significant reduction in labelling of GP I, II, III and actin. These changes were initiated 30″ after thrombin treatment and reached maximum after 3min. incubation with thrombin. Two new peptides were identified as having a surface orientation after thrombin treatment. The molecular weight of the two components were 80, 000 and 65, 000 respectively. Similar new peptides were demonstrated in inside labelled platelet membranes. No significant change was observed in membrane glycoprotein profile of thrombin treated platelet aggregates. Surface labelling pattern was never changed when thrombin induced platelet aggregation was inhibited either by the addition of 1mM EDTA or by no stirring. These results suggest that interaction of surface components of platelet membranes, particularly glycoproteins may involve the mechanism of early phase of platelet aggregation by thrombin.
- 一般社団法人 日本血栓止血学会の論文
一般社団法人 日本血栓止血学会 | 論文
- 日本人のＡＤＡＭＴＳ１３
- Infection of specific strains of Streptococcus mutans exacerbated intracerebral hemorrhage
- Transcriptional regulation of megakaryopoiesis and thrombopoiesis
- 新規血小板活性化受容体ＣＬＥＣ－２ その発見から今後の展望まで:その発見から今後の展望まで
- １．臨床血栓止血学オーバービュー