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Stimulants-induced changes in platelet membrane fluidity were observed by electron spin resonance (ESR) using spin probe (5SAL). The order parameter of stearate spin probe was markedly decreased when the washed platelets were aggregated by thrombin, collagen or Ca2+-ionophore A23187. However, isolated plasma membranes did not show any decrease in the order parameter by the addition of thrombin. After the platelets were treated with aspirin and colchicine which are potenti inhibitors for aggregation, the consequent addition of thrombin caused no significant increase in membrane fluidity. Moreover, any change in order parameter was not detected after treatment with N-ethylmaleimide which inhibited both aggregation and secretion. On the other hand, secretion induced by thimerosal or thrombin in the presence of EDTA (5mM) was accompanied with a decrease in the order parameter.By the addition of thrombin to the washed platelet suspension, some significant alterations were observed to occur in the phospholipid composition. The content of phosphatidylinositol was decreased after the 30 sec-stimulation by thrombin, with an accompanying increase in the diacylglycerol level. The 60 min-treatment of platelets by the drug caused decreases in phosphatidylcholine and phosphatidylethanolamine, resulting in increases in their corresponding lysophospholipids.When platelets were activated by thrombin and examined by electron microscopy after freeze-fracture, no change was observed in distribution of intramembranous particles. The distribution of cholesterol-filipin complexes formed in the platelet membranes including intracellular organelles was not different from that of the untreated platelets, thus implying that the cholesterol distribution was not affected by aggregation.These results suggest that the increase in platelet membrane fluidity might be caused by reorganization of membrane (lipid) constituents rather than their compositional modification.
- 一般社団法人 日本血栓止血学会の論文
一般社団法人 日本血栓止血学会 | 論文
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