Endotoxin-induced cytotoxicity and procoagulant activity of mouse bone marrow cells

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1. Bone marrow cells of mouse given endotoxin (LPS-cells) accerelated the plasma recalcification time (RCT) and partial thromboplastin time (PTT) of mouse or rabbit plasma. PTT was more sensitive than RCT. 2. A significant procoagulant activity of LPS-cells was demonstrated as early as 4hr and the peak was found 8-18hr after endotoxin. This peak was coincided with those of the cytotoxicity and the decrease of nucleated cell counts in marrow. 3. Either the procoagulant activity of LPS-cells of the mouse or the activity of mouse brain thromboplastin (MBT) was more marked in mouse plasma than in rabbit plasma. There would be a species specificity between the two animals, as to coagulation. 4. The two regression lines of the PTT on either the number of LPS-cells or the dose of MBT were nearly parallel. 5. Dose response regression of cytotoxicity or generation of procoagulant activity on concentration of endotoxin injected was significant. Cytotoxicity and procoagulant activity of LPS-cells were demonstrated even by injection of as little as 5μg endotoxin. 6. There existed a definite correlation between the cytotoxicity and procoagulant activity of LPS-cells (n=20, r=-0.8452, p<0.001).These findings suggested that procoagulant activity of LPS-cells is shown to be tissue thromboplastin in nature, and the migrated-cells in circulation play an important role in blood coagulation of endotoxicosis.
一般社団法人日本血栓止血学会の論文