Flow cytometric DNA analysis from endoscopical biopsy specimens in colorectal cancers.
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概要
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For the purpose of reliable evaluation of cellular DNA content in endoscopic biopsy specimens by flow cytometry, we compared the DNA content in 3, 6, 10 biopsy specimens obtained from 16 colorectal cancer patients. At this time, DNA index (D. I) and coefficient of variation (C. V) were evaluated from these DNA histogram. As for cell harvest from specimens, we could obtain (3.45 ± 2.75) × 10<SUP>4</SUP>, (6.10 ± 4.49) × 10<SUP>4</SUP> and (1.59 ± 0.94) × 10<SUP>5</SUP> cells when 3, 6 and 10 biopsy specimens were taken from one sample material respectively. C. V and D. I were stabilized in all cases and the distribution of DNA histgram had not been changed in aneuploid cases, regardless of the different number of biopsy specimens. And also there were close correlation between D. I of biopsy specimens and that of the same resected tissue (r=0.997). In our study, it was indicated that a reliable D. I determination by flow cytometry can be estimated sufficiently with 3 specimens as biopsy material.
- 社団法人 日本消化器内視鏡学会の論文
社団法人 日本消化器内視鏡学会 | 論文
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