Effect of Monoclonal Antibody to P-Selectin on Ischemia-Reperfusion Injury in Skin Flap
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概要
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Ingumal island flap models were produced in rats and the effects of anti-P-selectin monoclonal antibody (mAb) in reducing ischemic-reperfusion (I / R) injury were examined. Twenty-five Sprague-Dawley rats were divided into three groups : (1) an anti-P-selectin mAb-treated group (<I>n</I> =10) ; (2) a saline-treated control group (<I>n</I> =10) ; and (3) a sham group (<I>n</I> = 5) . In the anti-P-selectin mAb-treated group, the superficial inguinal arteries and veins were clamped for 9 h and anti-P-selectin mAb (0.20mg / kg, 0.5 mL, i.v.) was administered via the caudal vein 15 min before reperfusion. The control group was injected with physiological saline (0.5 mL, i.v.) 15 min before the start of reperfusion after the 9-h period of ischemia. In the sham group, the arteries and veins were clamped for 5 min only. Rats were observed for 7 days. Skin flaps in the three groups were compared for overall area of survival, as well as histologically. The mean area of skin flap survival in the control, mAb-treated, and sham groups was 18.3%, 90.3%, and 100%, respectively. Histological examination of skin flaps from the control group revealed indicators of inflammation, including marked infiltration of inflammatory cells, necrosis and edema. In the mAb-treated group, the inflammatory markers were significantly attenuated, with the histological picture in this group similar to that of the sham group. In conclusion, the anti-P-selectin mAb attenuated I / R injury, suggesting the possibility of using neutralizing antibodies to develop anti-inflammatory drugs.
- 昭和大学・昭和医学会の論文
昭和大学・昭和医学会 | 論文
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