Determination of 2-Aminoethanol in 2,2',2"-Nitrilotriethanol by Reversed-Phase HPLC-UV Using o-Phthalaldehyde as a Pre-Column Derivatizing Reagent

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A measurement method for determination of 2-aminoethanol in 2, 2′, 2″-nitrilotriethanol by reversed-phase high-performance liquid chromatography (HPLC) with an ultraviolet detector has been developed using o-phthalaldehyde as a pre-column derivatizing reagent. As a result, the calibration curve of 2-aminoethanol standard solutions was linear over the range of 1.0×10-5-5.0×10-3mol/L with a correlation coefficient of 0.996 and the detection limit, defined as S/N=3, was 7.5×10-7mol/L. The relative standard deviation at 5.0×10-5mol/L of 2-aminoethanol standard solution was 6.0% (n=6). This measurement method was applied to the determination of 2-aminoethanol in several commercial 2, 2′, 2″-nitrilotriethanol samples and it was found that both the calibration curve method and the standard addition method based on the peak height are available for the determination of 2-aminoethanol. These results suggest that the method reported here will be useful for determination of 2-aminoethanol in 2, 2′, 2″-nitrilotriethanol.
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