Studies for Identifying Prohibited Ingredients Such as Bithionol and Dichlorophen in Cosmetics.

概要

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Bithionol (BL) and dichlorophen (DF) are named in the Japanese Pharmaceutical Affairs Act as ingredients prohibited in cosmetics. So the methods for identifying BL and DF by HPLC were investigated. After adding BL or DF to lotion, milky lotion, body soap or shampoo and diluting with methanol, the extract was analyzed by HPLC using a Unisil Q C18 column (4.6mm i.d.×150mm), a mixture of acetonitrile and 50mM phosphate buffer (pH 3.5) (13:7) for BL or (1:1) for DF as the mobile phase and the detection wavelength of 306nm for BL and either 227nm or 286nm for DF, respectively. In the experiment curves for BL and for DF ranged from 1 to 10μg/mL and from 0.1 to 1.2 or 1 to 12μg/mL. There was good correlation between the concentration and the peak area of BL or DF. BL and DF added to lotion, milky lotion, body soap or shampoo-except for milky lotion D-were completely determined by HPLC. In the case of milky lotion D, an ingredient in that lotion interrupted the peak of DF on the HPLC chromatogram. So a pre-screening method with a short silica gel column was employed and the DF in milky lotion D was satisfactorily determined by HPLC.
日本化粧品技術者会の論文