Determination of Procaine Hydrochloride and Dibucaine Hydrochloride in Lotions, Milky Lotions and Creams by HPLC.

概要

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Procaine hydrochloride and dibucaine hydrochloride are commercially used as local anesthesia, but these ingredients are incompatible for cosmetics as per public comment of Ministry of Health and Welfare of Japanese Government in 1986 (ER-2 Notification No. 100 of March 12, 1986). In order to find the ingredients of incompatibility in cosmetics effectively and for the safety of cosmetics, we have estimated the determination method of procaine hydrochloride and dibucaine hydrochloride by HPLC and applied the method to the analysis of cosmetics. Procaine hydrochloride and dibucaine hydrochloride in the lotions or milky lotions or creams were extracted with water or methanol. The extract was analyzed by HPLC using the column (Shiseido CAPCELL PAK C18, 4.6×250mm), mixture of acetonitrile and 50mM phosphate buffer (pH 5) (37:3 or 65:35) were used as the mobile phase with the UV detector (detection wavelength: 296 or 228nm). By the use of the above analytical method we made it sure that procaine hydrochloride and dibucaine hydrochloride could be determined without the interference of the ingredients.
日本化粧品技術者会の論文