Determination of Estradiol and Ethinylestradiol in Milk Lotion and Cream.

概要

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The determination of estradiol and ethinylestradiol in cosmetic milk lotion and cream has already been established in the Standard Method of Analysis for Hygienic Chemist with Commentary 1995. But the harmful reagents against the human body such as chloroform and dichloromethane are used in the analytical procedures. In order to eliminate the harmful reagents and simultaneously protect the determination of estradiol and ethinylestradiol from the interference of parabens in the most part of cosmetics, we estimated a new and convenient method. The milk lotions or creams were condensed onto the water bath and then estradiol or ethinylestradiol in the residue was extracted with ethanol. The extract was analyzed by the high-performance liquid chromatography using the ODS column (TSKgel ODS80TM, 4.6×150mm), the mixture of water, acetonitrile and methanol (3:1:1) as a mobile phase and the fluorescence detector (excitation and emission wavelength: 290 and 310nm). By the use of the analytical method above we made sure that estradiol and ethinylestradiol could be determined without interference of parabens as conservatives or other ingredients.
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