Application of Semi-micro HPLC for Determination of Active Ingredients in Quasi-drug Products.
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概要
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Application of semi-micro HPLC which can reduce a solvent usage to approximately 1/10 in comparison with a conventional HPLC was studied for routine determination of active ingredients in quasi-drugs. Conditions established for the conventional HPLC was found to provide the same retention and separation when a linear velocity of carrier was kept constant. Reproducibility of a 2μl injection was excellent wish C.V.% of 1-2%. Analytical results of 6 active ingredients in hair grower by a complex gradient method were also found to be satisfactry.
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