Application of capsule-type packing materials for reversed-phase liquid chromatography in cosmetic analysis.

概要

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Octadecyl silyl (ODS-) silicagels, used widely as stationary phase in reversed phase liquid chromatography, possess problems such as stability in alkaline mobile phase, and peak tailing of basic substances due to silanol groups (Si-OH) on the silicagel surface.To overcome such problems, a reversed packing material differing fundamentally from the conventional ODS-silicagels was developed, by introducing Octadecyl groups into polymer coated silicagels. Structural elucidation by high resolution Solid-NMR, separation behaviors and alkaline stability are descussed. Furthermore, this packing material was used to examine the separation behaviors of glycosides, Mono-ammonium Glycyrrhizinate and Swertiamarin formulated in cosmetics were quantified.The newly developed packing materials showed separation behaviors basically resembling that of conventional ODS-silicagels. Since the packing material itself has become less polar, highly polar glycosides were found to elute more quickly with sharp separation. As regards to alkaline stability, no peak deterioration was observed after 300 hours of consecutive runs under pH=10.
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