Expression of nitric oxide synthase in human placenta in preeclampsia.

概要

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The purpose of this study was to determine whether any isoforms of nitric oxide synthase (NOS)in placenta are related to pathogenesis of preeclampsia. Two groups of placentas were studied; the control group of healthy pregnant women (n=3), the another of preeclamptic patients with severe hypertension (n=5). With Western blot analysis, endothelial NOS (eNOS) expression was detected in both of the groups. The imaging intensities of the enzyme found in the preeclamptic group were significantly lower than those in the control group (3.8±1.1mg-1 vs 5.7±0.4mg--1, p<0.05). Immunohistochemical localization of eNOS was noted in syncytiotrophoblast of the placenta in preeclampia. For control placenta, the protein was revealed to localize in a same manner as in the disease. However, with Northern blot analysis, there were no difference in expression of mRNA of the enzyme between the two groups. With neuronal NOS, by Northern blot analysis there were only slight expression in the both group placenta but by Western blot analysis, no expression in any placenta. With inducible NOS, there were no expression detected by Western blot analysis or by Northern blot analysis in any placenta. These data suggested that a relative deficiency in production of nitric oxide due to decreased activity of placental eNOS may contribute to the pathogenesis of preeclampsia. [Adv Obstet Gynecol 49 (3); 205-211, 1997 (H9.5)]
近畿産科婦人科学会の論文