Establishing an in vitro decidualization model in mice.

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The mechanism of implantation is not fully understood because of the complexity of cellular interactions between embryos and endometrial stromal cells (ESCs). Although an in vitro system of implantation may contribute to the understanding of such complex biological phenomena in vivo, it is ethically impossible to use human embryos in these experiments. Therefore, an in vitro model of mouse implantation must be used to understand the mechanism of implantation. As a first step, we tried to establish an in vitro model of decidualization. Mouse ESCs were collected and incubated in DMEM supplemented with 10 % FCS, estradiol (E, 0.1nM)and progesterone (P, 100nM). ESCs cultured with EP transformed into large and decidua-like cells, producing decidual protein such as desmin. Ultrastructurally, these cells developed abundant amounts of rough endoplasmic reticulum during the transformation. These findings indicate that mouse ESCs in culture respond to ovarian steroids and show morphological and functional changes that are similar to those associated with decidualization in vivo. Therefore, this culture system may serve as an in vitro model of mouse decidualization. [Adv Obstet Gynecol 49(3); 193-204, 1997 (H9.5)]
近畿産科婦人科学会の論文