A study of prolactin producing cells of human decidua in organ and monolayer cultures and ultrastructural localization of prolactin in human decidual cells.

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It has been suggested that human decidua synthesizes and secretes prolactin (PRL). In this study, the regulation of the production of decidual PRL and the ultrastructural localization of PRL in the human decidua were investigated. The concentration of PRL in a medium of decidua and chorion was significantly less than that in a medium of decidua alone in organ culture. hCG, prostaglandins and progesterone did not affect the production of decidual PRL. However, significantly lower concentrations of decidual PRL were observed when decidua was incubated in a medium with estradiol in the organ culture. The concentration of PRL in a cultured medium of the cells separated at 1.04 density from the decidual tissue was high and increased progressively over 6 days.<BR>However, no significantly lower concentration of PRL was observed when the separated PRL producing cells were incubated with estradiol. Immunoelectronmicroscopic observations revealed that immunoreactive gold particles were located specifically on the intracytoplasmic small granules of decidual cells of 5 weeks of gestation. [Adv Obster Gynecol 47(2) : 287-299, 1995 (H7.3)]
近畿産科婦人科学会の論文