Study of the Automatic Screening of Ccrvical Seraping Smears Using a Folw Cytometer-EPICS C. (the Second Report). the Results of Measurement of 416 Smears.:the Results of Measurement of 416 Smears
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概要
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This present investigation is to see if a flow cytometer can automatically distinguish whether cervical scraping smears from mass screening are clearly negative or suspect.<BR>The flow cytometer, EPICS C was used throughout the trial.<BR>Each smear was doubly stained with propidium iodide and fluorescein isothiocyanate and then measured by EPICS C. The judgement as to wheter the smears were negative or not, was done by analyzing th pattern of 2 parameters cytogram of red and green fluorescence intensity (logarithm).<BR>In cytograms of the smears of typical benign cases, the 4 areas where squamous epithelial cells, neutrophiles, bacteria and dust respectively disseminate are clearly distinguishable. On the other hand, in cytograms of the smears of typical cervical cancer cases, these four dissminating areas fuse with each other and become an irregular mass.<BR>However, in benign cases with a number of benign atypical cells in the smear, cytograms of the smears may sometimes reveal patterns similar to that of malignant cases. Also, in malignant cases with only a few cancer cells in the smear, cytograms of the smears may sometimes reveal patterns similar to that of benign cases.<BR>Therefore, the precise standard of judgement of negative or suspect smears needs to be established.<BR>The results of 416 smears diagnosed by EPICS C using this new standard of judgement were compared with results obtained by Papanicolaou stained smears made from the same samples. As a consequence, the data was very nearly the same as that of Papanicolaou stained smears.<BR>It is desirable to attain as a first purpose, the use of EPICS C for automatic screening of cervical scraping smears by measuring many more smears and establishing a correct standard of judgement for EPICS C measurement of smears of cervical scraping.<BR>
- 近畿産科婦人科学会の論文
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