Androstenedione aromatase activity in adipose tissues of young women.

概要

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It has been reported that adipose tissues are able to aromatize androstenedione to estrone as one of the extragonadal tissues in humans and that they play an important physiological role instead of ovary in postmenopausal women. In this study, the authors examined the aromatase activity for androstenedione in adipose tissues from young women with prematured ovaries. The abdominal adipose tissue homogenate (1g w. w.) was incubated with [1, 2, 6, 7-3H] -androstenedione (118pmol) and NADPH (10mg) at 37°C for 1 hour in air. After stopping the enzyme reaction, [4-14C] -estrone and [4-14C] -estradiol (1×104dpm, 250μg, respectively) were added in the incubated sample. The extract with ethyl acetate was submitted to Bio-Rad AG1-X2 column, thin layer chromatographies and co- crystallization to constant specific activity and 3H/14C ratio. Estrone was formed in all samples (53-98fmol/g/h), but estradiol was formed in 2 of 5 samples. The level of estrogen biosynthesis in adipose tissues of young women was not changeable compared to it in the sexual matured women.
近畿産科婦人科学会の論文