ヒト絨毛性ゴナドトロピン(hCG)と絨毛性FSH(hCFSH)の卵胞顆粒膜細胞への作用
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Biological properties of human chorionic gonadotropin (hCG) and human chorionic follicle stimulating hormone (hCFSH) were investigated using porcine granulosa cells. Specific binding of radioiodinated hCG and hCFSH to granulosa cell suspension increased in the course of incubation through 120 min. The binding ability of 125I-hCFSH to the cells was greater than that of 125I-hCG. Increasing concentrations of unlabeled hCG in the incubation mixture progressively inhibited the uptake of 125I-hCG by granulosa cells, whereas unlabeled hCFSH was not able to compete with 125I-hCG binding. On the other hand, unlabeled hCFSH competed with the binding of 125I-hCFSH to granulosa cells, while unlabeled hCG did not compete with 125I-hCFSH binding. In the course of culture of granulosa cells, the binding ability of 125I-hCFSH was markedly reduced, while that of 125I-hCG was retained in the same level.Granulosa cells harvested from large follicles and medium follicles grew in an epithelioid mann er after the culture for 3 days. Progesterone level in the culture medium increased gradually in the course of the culture of granulosa cells. Addition of hCG into the culture medium markedly stimulated progesterone secretion by the granulosa cells in culture after 6 hr of incubation, whereas hCFSH showed a slight increase in progesterone secretion after that time period. In addition, incubation of porcine granulosa cells with hCG or hCFSH resulted in augmentation of cyclic AMP content. The stimulation of c-AMP production in granulosa cells was found within 30 min following addition of these hormones and the c-AMP levels remained elevated for 3 hr. The stimulatory effect of hCG on c-AMP production was greater than that of hCFSH. In 30 min incubation, the maximal stimulation of c-AMP production in granulosa cells was produced with 1μg hCG or hCFSH, while the minimum effective dose of hCG or hCFSH was 0.01μg. Moreover, the similar concentrations of hCG or hCFSH activated protein kinase in granulosa cells. It can be concluded from these results that porcine granulosa cells have two different types of receptor sites: one for hCG and the other for hCFSH and that the binding ability for hCFSH disappear in the course of lutenization of the granulosa cells, whereas that for hCG retained. Furthermore, the main biological effect of hCG and hCFSH upon the porcine granulosa cells is stimulation of steroidogenesis and folliculogenesis, respectively. It is highly suggested that the hormonal action of hCFSH may be mediated by adenyl cyclase-cyclic AMP system in a similar manner to the hormonal action of hCG.
- 近畿産科婦人科学会の論文
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