Studies on Amniotic Fluid Cell Culture and Chromosome Analysis for Prenatal Diagnosis

概要

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Culture of amniotic fluid cells is indispensable to the diagnosis of fetal genetic disorders. But, most of the procedures reported have been time consuming and have had relatively low success rate. In this study, factors influencing on amniotic fluid cell growth in culture were clarified and their optimum conditions were examined.On the bases of the results obtained, a successful culture method using maternal serum was established.Sanguineous samples were treated with hypotonic solusion in order to eliminate undesirable action of contaminating blood cells. Fathermore, in the cases of glossly bloody tap, fetal karyotyping was successfully made by using fetal lymphocytes from placental puncture.In order to obtain optimum numbers of cells for chromosome analysis, chromosome preparations were made by in situ method without trypsinization, and a half-drying fixation method prior to fixatives was developed to prevent the mitotic cells from being detached.From these experimental results, the success rate was improved and the time taken to make chromosome preparations was shortened. As a result, prenatal diagnosis using amniotic fluid cells became more reliable and rapid in practice
近畿産科婦人科学会の論文