The Application of Centrifugal Analyzer to EMIT Assay of Five Antiepileptic Drugs
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The application of centrifugal analyzer to EMIT is worth-while to assay quickly many specimens and to minimize the expenses of the reagents for each specimen.An attempt to apply centrifugal analyzer to five drugs (phenytoin, phenobarbital, primidone, carbamazepine and ethosuximide) and to obtain directly the computerized data of concentration was performed for the practical use of centrifugal analyzer.<BR>The temperature differences between cells in the disc of the analyzers (Gemsaec N III and Rotochem II) were eramind by cresol red method, and were found to be within±0.015°C.<BR>In using centrifugal analyzer the EMIT reagents were diluted based on the method of Finley et al., while both delay-time and reaction-time were chosen to be 100 seconds for all five drugs.The standard curves for phenytoin, primidone and carbamazepine on bilogarithmic diagram were straight lines, while those of phenobarbital and ethosuximide revealed nonlinear curves.Therefore, in order to obtain computerized data of concentratic equadratic equation and logit transformation were found to be acceptable for all five drugs. The precision of the EMIT assay using the procedure described here was at the level from 2.7 to 5.3% (between disc) and from 2.1 to 4.5% (within disc), expressed as coefficient of variation.<BR>With this analytical system for the patient sera, the correlation coefficient of EMIT manual vs centrifugal procedures wes at the level from 0.962 to 0.990 for five drugs.This was similar to the previously reported values of the correlationship between GC and EMIT manual procedures, that is from 0.940 to 0.991.As a matter of fact the centrifugal procedure described hese seems to be as dependable as GC, and is more rapid and less expensive than EMIT manual procedure.
- 一般社団法人 日本小児神経学会の論文
一般社団法人 日本小児神経学会 | 論文
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