Production of beta 2 microglobulin in vivo and in vitro in maintenance dialysis patients.

概要

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Production of beta 2 microglobulin (B2M) was studied in vivo and in vitro in mainten ance dialysis patients. Changes in serum B2M concentration was examined before and after hemodialysis with three different types of dialyzer membrane. Synthesis of B2M was also studied on cultured mononuclear cells. Plasma B2M concentration was significantly increased at the end of dialysis using cuprophane or PMMA; B2 membrane dialyzer, while a significant reduction was noticed in EVAL membrane dialyzer. These changes in plasma B2M after dialysis seems to depend on the removal capacity of dialyzer and not to depend on complement activation. B2M synthesis of cultured mononuclear cells was significantly decreased in maintenace hemodialysis patients compared to normal volunteers, however, there was no change in CAPD patients. Ex vivo recirculation of heparinized whole uremic blood enhanced B2M synthesis on mononuclear cells, which did not relate to types of sterilization method. Those changes may partly be respohsible for abnormal metabolism of B2M in chronic dialysis patients.