Bioseparation of Lectin by Dextran Produced by Dextransucrase

概要

論文の詳細を見る
Dextransucrase (DSase) forms a complex with dextran during reaction with sucrose as a substrate. Dextransucrase-containing solution was permeated through the inorganic membrane of Shirasu Porous Glass to immobilize the enzyme on the pore surface. Sucrose solution (25 g/L) was again permeated through the dextransucrase-immobilized membrane, producing the dextran throughout the pore at the range to 15 mg/g. Concanavalin A extracted from jack bean was adsorbed to the produced dextran in permeation mode. Concanavalin A adsorption was evaluated by changing the amount of produced dextran and the residence time of concanavalin A in the membrane.
日本イオン交換学会の論文