Dynamic light scattering study of the conformational change of ovotransferrin induced by the binding of lanthanoid ions.
スポンサーリンク
概要
- 論文の詳細を見る
In order to elucidate the predominant factors for the confo rmational change of chicken ovotransferrin (OTf), which has one metal-binding site in each of amino (N) and carboxyl (C) terminal domains, induced by, metal binding, the effects of the coordinated metal ions on the diffusion coefficients (D) of the metal-OTf complexes were investigated by means of dynamic light scattering technique using various lanthanoid (Ln<SUP>3+</SUP>) ions with a high affinity for C metal-binding site. The D values of the OTf complexes increased with increasing amounts of bound Ln<SUP>3+</SUP> up to (Ln<SUP>3+</SUP>)/(0Tf) (=R) =1.2, and then gradually changed, leveling off above R=2, regardless of the species of Ln<SUP>3+</SUP> metals. The behavior of D values with increasing degree of saturation of bound metals was related to that of the C-site complexes compared to the N-site complexes. In addition, the D values of the saturated OTf complexes incredsed with decreasing ionic radii of Ln<SUP>3+</SUP>. These results suggested that the sto kes radii of the OTf complexes appreciably depend on the ionic radii of bound Ln<SUP>3+</SUP> and that the contraction of the OTf molecules originates from a predominant metal binding to the C-site compared to the N-site.
- 公益社団法人 日本化学会の論文
公益社団法人 日本化学会 | 論文
- Spectrophotometric Studies on the Kinetics of Decomposition of Tetramethylmurexide in Acid Medium
- The Crystal Structures of Metal 8-Hydroxyquinolinate. I. Copper 8-Hydroxyquinolinate
- Hydrophile-Lipophile Balance Values for O/W Emulsions Stabilized by Nonionic Surfactants. I. Hydrophile-Lipophile Balance Values of Nonionic Surfactants Determined by Emulsification
- The Formation of Acetylenic Bond by the Elimination Reaction of Some Enol-esters. III. Formation of α, β-Acetylenic Carbonyl Compounds
- Ultraviolet Absorption Spectra of Lead Aldose-cysteinate