Functional Domain Analysis of Human HP1 Isoforms in Drosophila

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Three subtypes of HP1, a conserved non-histone chromosomal protein enriched in heterochromatin, have been identified in humans, HP1α, β and γ. In the present study, we utilized a Drosophila system to characterize human HP1 functions. Over-expression of HP1β in eye imaginal discs caused abnormally patterned eyes, with reduced numbers of ommatidia, and over-expression of HP1γ in wing imaginal discs caused abnormal wings, in which L4 veins were gapped. These phenotypes were specific to the HP1 subtypes and appear to reflect suppressed gene expression. To determine the molecular domains of HP1 required for each specific phenotype, we constructed a series of chimeric molecules with HP1β and HP1γ. Our data show that the C-terminal chromo shadow domain (CSD) of HP1γ is necessary for HP1γ-type phenotype, whereas for the HP1β-type phenotype both the chromo domain and the CSD are required. These results suggest human HP1 subtypes use different domains to suppress gene expression in Drosophila cells.
日本細胞生物学会の論文