Detection of Type C Insulin-Resistant Patients by an Enzyme-Linked Immunosorbent Assay of Autophosphorylated Insulin Receptors.
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概要
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The erythrocyte insulin receptors of 16 patients with basal hyperinsulinemia were tested for their ability to autophosphorylate. The assay was performed using a new enzyme-linked immunosorbent assay (ELISA) system utilizing monoclonal anti-insulin receptor antibody absorbed to microtiter plates as a first antibody and polyclonal anti-phosphotyrosine antibody as a labeled second antibody. By means of this assay, three patients were identified as having defects in their insulin receptors, although these defects appeared heterogeneous. Patient No.1, with acanthosis nigricans, had normal maximal autophosphorylation, but the ED<SUB>50</SUB> was shifted to the right (2.9×10<SUP>-8</SUP>M, control 1.6×10<SUP>-9</SUP>M). Patient No.2, with polycystic ovary disease, had a 49.2% decrease in maximal insulin receptor autophosphorylation, and the ED<SUB>50</SUB> was shifted to the right (5.6×10<SUP>-9</SUP>M). Patient No.3 had 85% less maximal autophosphorylation with a normal ED<SUB>50</SUB>. The mechanism (s) for the diversity detected in this assay is (are) not known, but this technique has sufficient specificity and sensitivity to be used to screen for patients resistant to insulin due to a lack of kinase activity.
- 一般社団法人 日本糖尿病学会の論文
一般社団法人 日本糖尿病学会 | 論文
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